Kits For DNA Recovery using digital PCR

Analysis of bisulfite kits for DNA methylation profiling by way of DNA fragmentation and DNA restoration utilizing digital PCR.

DNA methylation is among the most vital epigenetic modifications within the regulation of gene transcription.

The present gold customary to check this modification is bisulfite sequencing. Though a number of industrial bisulfite remedy kits present good conversion efficiencies, DNA loss and particularly DNA fragmentation stay troublesome.

This hampers DNA methylation profiling of lengthy DNA sequences. Right here, we explored the efficiency of twelve industrial bisulfite kits by an in-depth comparability of DNA fragmentation utilizing gel electrophoresis, qPCR and digital PCR, DNA restoration by spectroscopic measurements, and digital PCR and conversion effectivity by next-generation sequencing.

The outcomes present a transparent efficiency distinction between the bisulfite kits, and relying on the precise purpose of the examine, probably the most acceptable equipment may differ.

qPCR plate-foil common (for Actual-time PCR, Mild Cycler 480)

Furthermore, we demonstrated that digital PCR is a beneficial methodology to observe each DNA fragmentation in addition to DNA restoration after bisulfite remedy.

ALV Real-Time PCR Kit
PD65-15	Bionote	96t	EUR 1724.4
Description: Please check the datasheet of ALV Real-Time PCR Kit before using the test.

0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR
PCR-2CP-RT-C	CORNING	125/pk	EUR 223
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

hsa-mir-521 Real-time RT-PCR Detection Kit
20-abx097600	Abbexa	EUR 551.00 EUR 787.00 EUR 398.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

dsGreen for Real-Time PCR, 100×, 100 uL
11010	lumiprobe	100 uL	EUR 59

dsGreen for Real-Time PCR, 100×, 2 mL
61010	lumiprobe	2 mL	EUR 363

dsGreen for Real-Time PCR, 100×, 1.5 mL
51010	lumiprobe	1.5 mL	EUR 331

dsGreen for Real-Time PCR, 100×, 1 mL
41010	lumiprobe	1 mL	EUR 249

dsGreen for Real-Time PCR, 100×, 10 mL
81010	lumiprobe	10 mL	EUR 869

dsGreen for Real-Time PCR, 100×, 5 mL
71010	lumiprobe	5 mL	EUR 625

dsGreen for Real-Time PCR, 100×, 50 mL
91010	lumiprobe	50 mL	EUR 3169

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions	BosterBio	100 Reactions	EUR 327

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA)
31033	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2)
31034	Biotium	1kit	EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (nuc)
31035	Biotium	1kit	EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA)
31036	Biotium	1kit	EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA)
31050	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly)
31051	Biotium	1kit	EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)
31053	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx
31033-X	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx
31050-X	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx
31051-X	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit
RR-0478-02	Liferiver	25 tests/kit	EUR 991
For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycl Show more
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276)
31037	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2H	Detroit R&D	1 Kit	EUR 430

Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2M	Detroit R&D	1 Kit	EUR 430

Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2R	Detroit R&D	1 Kit	EUR 430

hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit
20-abx097601	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 266

Human Lung Cancer PCR Primer Library
HLUCPL-I	Real Time Primers	1 set	EUR 548

Human Schwann Cell PCR Primer Library
HSCH-I	Real Time Primers	1 set	EUR 548

qPCR plate-foil universal (for Real-time PCR, Light Cycler 480)
A26979	GeneOn	1x100 pcs	EUR 186

Eztime Real - time PCRPremix 2x,Taqman
FYT105-100P	Yeastern Biotech	100 Preps 1.25 ml	Ask for price

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx
31037-X	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-100P	Yeastern Biotech	100 Preps 1.25 ml	Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-400P	Yeastern Biotech	400 Preps 1.25 ml x 4	Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX
FYT104-100P	Yeastern Biotech	100 Preps 1.25 ml	Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX
FYT104-400P	Yeastern Biotech	400 Preps 1.25 ml x 4	Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX
FYT105-400P	Yeastern Biotech	400 Preps 1.25 ml x 4	Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX
FYT106-100P	Yeastern Biotech	100 Preps 1.25 ml	Ask for price

0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS
PCR-0108-LP-RT-C	CORNING	125/pk	EUR 709
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS
PCR-0108-LP-RT-W	CORNING	125/pk	EUR 770
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097603	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097604	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097605	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097606	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097607	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097608	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097609	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097610	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097611	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097612	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097613	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097614	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097615	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097616	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097617	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097618	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097619	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097620	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097622	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097625	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097626	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097627	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097628	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097629	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097630	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097631	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097632	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097633	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097634	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097636	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097637	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

Developmental validation of the DNATyper™Y26 PCR amplification equipment: An enhanced Y-STR multiplex for familial looking out.

The DNATyper™Y26 PCR Amplification equipment, which together with 26 low-medium mutating Y-STRs, is designed for Y-STR familial looking out casework. The equipment combines 9 new Y-STR loci along with the 17 Y-STR loci from the commercially obtainable AmpFlSTR®Yfiler® equipment.

The validation of the DNATyper™Y26 equipment was carried out by way of technical index, together with accuracy, stability, species specificity, sensitivity, adaptability for varied samples, and combination.
Additional, mutations of the 26 Y-STRs had been analyzed by 1167 DNA-confirmed father-son pairs, and the outcomes indicated that these loci had low or medium mutation charges.
Moreover, these Y-STRs loci had been additionally examined in 1072 random male samples from Henan, Shanxi, Inside Mongolia, and Chongqing in China, exhibiting their excessive energy for forensic discrimination within the Chinese language inhabitants.
Thus, the DNATyper™Y26 PCR Amplification equipment is a robust instrument for ‘Y-STRs familial looking out’ in precise sexual-assault circumstances, indicating its distinctive benefit in familial looking out attributable to Y-STR loci with solely low-medium mutation charges.

Human Lung Most cancers PCR Primer Library

The industrial PowerPlex® Fusion equipment is an autosomal STR multiplex equipment that has excessive discrimination energy and is extra informative in forensic, paternity, and relationship-testing circumstances.

Key options of this multiplex system are the likelihood to direct amplify FTA™ card punches in addition to non-FTA playing cards and generally used swabs; optimised inhibitor tolerance and excessive sensitivity producing full profiles from quantity as little as 100 pg of human DNA.

This examine targeted on the optimization of efficiency variables equivalent to FTA™ punch sizes, diminished response volumes, and FTA™ purification reagent aiming to extend the analytical sensitivity, lower the pattern consumption, and price effectiveness.

Eztime Actual – time PCRPremix 2x,SYBR Inexperienced

LOD and LOQ values demonstrated excessive sensitivity of the PowerPlex® Fusion system. As well as, inhabitants databases of Brunei Malay and Chinese language from the Brunei Darussalam had been established, and parameters of forensic significance had been calculated.

General, the forensic parameters indicated an enhanced utility of the PowerPlex® Fusion equipment for forensic proof evaluation and paternity testing in Brunei Malay and Chinese language populations.

PCR Mycoplasma Detection Package

Diagnosing of influenza quickly and precisely helps clinicians to provoke acceptable remedy choices and isolation protocols. Unnecessary antimicrobial remedy and laboratory testing will also be diminished. Assess industrial alternate options to in-house assays that will not solely cut back laboratory technician “palms on” time but in addition the laboratory turnaround time is of curiosity.

We evaluated the efficiency of the VIASURE Flu A, B & RSV Actual Time RT-PCR Detection Package (CerTest Biotec) for detecting Influenza A and B viruses.

In the course of the 2016/17 influenza season 532 scientific samples had been examined with the VIASURE assay on the BD MAX™ system versus an in-house actual time RT-PCR assay with discrepant outcomes resolved by an actual time RT-PCR assay at a nationwide reference laboratory.

The VIASURE assay on the BD MAX confirmed a sensitivity of 99.5% (95% CI: 97.3-100) and a specificity of 99.1% (95% CI: 97.4-99.8) for detection of Influenza A virus. The optimistic predictive and detrimental predictive values had been 98.5% (95% CI: 95.8-99.7) and 99.7% (95% CI: 98.3-100) respectively. Influenza B virus detection couldn’t be evaluated attributable to a low positivity price. The BD MAX platform provided the flexibleness of a number of each day runs, shorter hands-on-time and shorter turnaround time than the in-house assay.

The VIASURE assay on the BD MAX carried out properly and is now applied in our scientific laboratory.

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